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Interaction of Bicalutamide and Radiation in Androgen-Dependent and Androgen-Independent Prostate Carcinoma Cells

Issue: Vol.2, No.2 - January 2003

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Article Type: Manuscript

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  1. Dr James A. Bonner
    MD
    University of Alabama at Birmingham, Department of Radiation Oncology,
  2. Michael Vander Kooy
  3. Teresa Christianson
  4. A Ahmed
  5. Donald Tindall

Purpose: This project was undertaken to determine whether the known bicalutamide-induced growth arrest properties are associated with entry of prostate carcinoma cells into a cell cycle phase that may impact radio-sensitivity.

Materials and Methods: An androgen-independent (PC3 Neo) cell line and its counterpart transfected with the androgen receptor (PC3AR) were used for these studies. Cell survival was assessed by colony formation, and cell cycle distributions were assessed by flow cytometry.

Results and Conclusions: Exposure to bicalutamide (100 g/ml) produced a progressive accumulation of PC3AR cells in G1, with the maximum increase after 10 hours of exposure (140% of controls). Various pre-radiation exposures (2-24 hours) to bicalutamide did not result in any significant change in baseline radio-sensitivity of the PC3 Neo or PC3AR cells.

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