Nucleolar Organizer Regions in Squamous Cell Carcinomas of the Uterine Cervix Treated with Chemoradiotherapy

Issue: Vol.8, No.2 - April 2009

« Back to Articles

Article Type: Manuscript

Download PDF Version »

  1. Dr Takashi Nakano
    Department of Radiation Oncology, Gunma University Graduate School of Medicine
  2. Lin Kurnia
  3. Yoshiyuki Suzuki
  4. Budiningsih Siregar
  5. Andri Andrijono
  6. Irwan Ramli
  7. Cholid Badri
  8. Yukari Yoshida

Background: Nucleolar organizer regions (NORs) are chromosomal loops of DNA involved in ribosomal synthesis. Their size and number in a nucleus has been reported to reflect the proliferative activities of various cells. The purpose of this study was to assess the meaning of the number of NORs in tumor-cell nucleoli compared with other proliferative markers before and after 10 Gy irradiation.

Materials and Methods: Forty six biopsy specimens from twenty three patients with cervical squamous cell carcinoma treated with chemo-radiotherapy at Cipto Mangun Kusumo Hospital (Indonesia) taken before radiotherapy and after exposure to 10 Gy were analyzed. The number of NORs was measured using a silver staining method. MIB-1 and p53 labeling indexes (LI) were measured using an immunohistochemical method. Histological radiation response and mitotic index (MI) were investigated with hematoxylin and eosin staining.

Result: After 10 Gy irradiation, the number of NORs per nucleolus (AgNOR score) decreased from 4.9 to 3.4 (p<0.001). MIB-1-LI and MI increased from 26 to 39 (p<0.01) and from 0.006 to 0.012 (p<0.001), respectively.
Before radiation therapy, a positive correlation was found between AgNOR score and MIB-1-LI (p=0.001) and between AgNOR score and MI (p=0.01). At 10 Gy, there was no significant correlation among number of NORs, MIB-1-LI,
MI, or p53-LI. Histological radiation response had no correlation with AgNOR, MIB-1-LI, MI, or p53-LI.

Conclusion: The number of NORs can be used as proliferative marker for assessing tumor cell proliferative activity before chemo-radiotherapy but not after 10 Gy irradiation.

Download PDF Version »

Favourites   Share / Bookmark

Also In This Issue

« Back to Articles